The Bowden Laboratory is equipped with several state-of-the art instruments and sample processing/extraction equipment.
For Fee for Service options, see here
For Fee for Service options, see here
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The Thermo TSQ Quantis triple quadrupole mass spectrometer is ideal for targeted analyses of known compounds (e.g., personal-care products, pharmaceuticals environmental pollutants, hormones). This triple-quadrupole mass spectrometer offers very high sensitivity and robustness, with some of our methods having limits of detection in the low parts per trillion. Most studies performed using this instrument are hypothesis testing, using selected reaction monitoring scan mode to identify components in the sample. We are currently coupling 96-well plate technology to our current targeted assays.
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Typically used in discovery studies (hypothesis driving experiments), the Thermo Q-Exactive quadrupole-orbitrap hybrid mass spectrometer is capable of high-resolution analyses (up to 140,000 at m/z 200), sub-ppm mass accuracy with lock-mass correction and MS/MS with higher-energy collision-induced dissociation. This instrument is currently used in -omic studies, specifically lipidomics and metabolomics for pathway elucidation and the discovery of novel compounds as markers of disease, environmental exposure, and diet. We are also developing methods for the nontargeted analysis of per- and polyfluoroalkyl substances and chemicals of emerging concern in the environment.
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The laboratory has a Thermo Scientific Exploris Gas Chromatograph 240 Orbitrap Mass Spectrometer coupled to a Thermo Trace 1310 gas chromatograph. The instrument provides enhanced sensitivity, selectivity, and confirmation of known and unknown compounds via retention time matching, accurate mass information of both the parent and fragment ions, and isotope patterns. The instrument is used (in electron, positive chemical, and chemical ionization mode) for exploratory analysis, to provide a more complete characterization of GC-amenable chemicals, like volatile PFAS in complex samples. Data is processed using Compound Discoverer and spectral matching with high resolution databases.
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For sample processing and extraction, the laboratory has several resources, including a Thermo ExtrevaASE (retrofitted for PFAS extraction), a SPEX 6875D cryomill, a PromoChrome SPE-03 automated SPE manifold, four traditional SPE manifolds, grinders and dehydrators, a Biotage TurboVap, 96-well plate technology, as well as several -20 and -80 C freezers.
The laboratory also has dedicated PFAS-free field sampling equipment capable of collecting both liquid and solid matrices, as well as sediment cores. Dr. Bowden also has a YSI Professional Series water chemistry probe. |
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